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Antibody specificity against NDRG family proteins. Lysates of COS-1 cells transfected with green fluorescent protein (GFP) or mouse NDRG–GFP vectors were analyzed by Western blotting. Each antibody specifically reacted with respective NDRG–GFP fusion proteins (closed arrowheads). Bands indicated by open arrowheads were endogenous NDRG proteins. Each lane contained 5 μg of total protein.
