Localization of histo-blood group A type 3 antigens reactive to AR-1, and H type 3/4 antigens reactive to MBr1 in normal skin. A type 3 antigens are localized in dark cells (arrows) and duct epithelial cells (arrowheads) in eccrine sweat glands in specimen from A blood group secretor (A,B). Only duct epithelial cells of eccrine sweat glands were reactive to AR-1 in specimens from non-secretors (C,D). Histo-blood group H type 3/4 antigens were detected in dark cells but not duct cells of eccrine sweat glands in specimens from O blood group secretors (E,F). Most vascular endothelial cells did not express type 3 antigens (large arrows). Areas indicated by boxes in A, C, and E are depicted at higher magnification in B, D, and F, respectively. Two sections from a wound specimen (Group II) were stained with AR-1 absorbed with blood group A (G) and O red cells (H), respectively. Absorption with blood group A red cells abolished reactivity of AR-1 (G). Bar = 50 μm.