Table 2.
Human brain material in tissue array B
| Donor
|
Fixation
|
|||||
|---|---|---|---|---|---|---|
| Number | Age (days) | Sex | PMI (days) | Time | Fixative | Autopsy diagnosis/death conditions |
| 1 | 63 | M | <1 | 8 days | 4% Lillies | Pneumonia, NIDDM |
| 2a | 86 | M | 2½ | 2 weeksa | 4% Lillies | NA |
| 2b | 86 | M | 2½ | 2 yearsb | 0.1% PFA | NA |
| 3a | — | M | 1 | 2 weeksa | 4% PFA | NA |
| 3b | — | M | 1 | 2½ yearsb | 0.1% PFA | NA |
| 4a | 82 | M | 1 | 2 weeksa | 4% PFA | Heart failure, coronary heart disease |
| 4b | 82 | M | 1 | 2½ yearsb | 0.1% PFA | Heart failure, coronary heart disease |
| 5a | 73 | M | <1 | 2 weeksa | 4% PFA | Respiratory and circulatory arrest after surgery |
| 5b | 73 | M | <1 | 3 yearsb | 0.1% PFA | Respiratory and circulatory arrest after surgery |
| 6a | 59 | M | 2 | 2 weeksa | 4% PFA | Heart failure after AMI |
| 6b | 59 | M | 2 | 3 yearsb | 0.1% PFA | Heart failure after AMI |
| 7a | 88 | F | 2 | 3 weeks | 4% PFA | Heart failure, ischemic heart disease |
| 7b | 88 | F | 2 | 4 yearsb | 0.1% PFA | Heart failure, ischemic heart disease |
| 8 | 71 | F | <1 | 2½ months | 4% Lillies | COLD, hypertension, hypothyroidism |
| 9 | 61 | M | <1 | 3 months | 4% Lillies | Circulatory failure, cancer, IDDM |
| 10 | 70 | M | <1 | 3 months | 4% Lillies | Heart failure, hypertension |
| 11 | 76 | M | 4 | 3 months | 4% Lillies | Acute gastrointestinal bleeding |
| 12 | 65 | M | 4 | 3 months | 4% Lillies | Pneumonia, history of alcoholism |
| 13 | 40 | F | NA | 3 months | 4% Lillies | Lung stasis and atherosclerosis, heart failure |
| 14 | 72 | M | 1 | 3½ months | 4% Lillies | Pneumonia, duodenal ulcus, history of alcoholism |
| 15 | 55 | M | 1 | 3½ months | 4% Lillies | Acute gastrointestinal bleeding |
| 16 | 75 | M | 2½ | 3½ months | 4% Lillies | Cancer |
| 16 | 31 | F | 1 | 3¾ months | 4% Lillies | Leukemia |
| 18 | 88 | F | 6 | 4 months | 4% Lillies | Circulatory failure after femoral fracture |
| 19 | 81 | M | 5 | 4 months | 4% Lillies | Multiorgan failure, history of alcoholism |
| 20 | 88 | F | 3 | 4 months | 4% Lillies | Heart failure |
| 21 | 79 | M | NA | 2 years | 4% Lillies | NA |
| 22 | 79 | M | NA | 2 years | 4% Lillies | NA |
| 23 | 86 | M | 2 | 8 years | 4% Lillies | Respiratory and circulatory failure, lung cancer |
| 24 | 76 | F | 2 | 8 years | 4% Lillies | Heart failure/AMI |
| 25 | 78 | M | 3 | 9½ years | 4% Lillies | Respiratory and circulatory failure, asthma attack |
| 26 | 84 | F | 1 | 9½ years | 4% Lillies | Heart failure, COLD |
| 27 | 81 | M | NA | 10 years | 4% Lillies | Ulcus ventriculi |
| 28 | 93 | F | NA | 10 years | 4% Lillies | Pulmonary emboli |
| 29 | 74 | M | 2 | 10 years | 4% Lillies | Respiratory and circulatory failure after surgery |
The donor was perfused with 4% Lillies PBFS through the internal carotid artery before removal of the brain from the skull, and the brain was immersed in 4% PFA solution.
The material was initially fixed by perfusion and immersion as indicated in Donor #a, but was kept in 0.1% PFA solution at 4C for long-term storage.
Specimens in tissue array B were sampled from the postcentral gyrus and embedded in paraffin. Donor numbers marked with a and b indicate that the samples are from the same brain at different stages of fixation. Donors 20 and 28 were the same as Donors 9 and 10 in tissue array A. NIDDM, non–insulin-dependent diabetes mellitus; AMI, acute myocardial infarction; COLD, chronic obstructive lung disease; IDDM, insulin-dependent diabetes mellitus; PBFS, phosphate-buffered formalin solution.