Table 3.
Primary antibodies
| Antigen (clone) | Type | Cat. no. | Supplier | References | Notes |
|---|---|---|---|---|---|
| β-tubulin III (Tuj1) | Ms IgG2a | 01409 | Stemcell Techn. | Geisert and Frankfurter (1989) | |
| Menezes and Luskin (1994) | |||||
| CD11b/C3bi-R (2LPM19c) | Ms IgG1 | M0741 | Dako Cytomation | Wright et al. (1988) | |
| Dominguez et al. (2001) | |||||
| CD14 (7) | Ms IgG2a | CD14-223 | Novocastra | Wright et al. (1991) | a |
| Ziegler-Heitbrock et al. (1994) | |||||
| CD34 (QBEnd/10) | Ms IgG1 | NCL-END | Novocastra | Anthony and Ramani (1991) | a |
| Krause et al. (1996) | |||||
| CD39 (A1) | Ms IgG1 | MCA1268 | AbD Serotec | Aversa et al. (1988) | |
| Wang and Guidotti (1998) | |||||
| CD45/ LCA (2B11/ PD7/26) | Ms IgG1 | M0701 | Dako Cytomation | Kurtin and Pinkus (1985) | a,b |
| Sasaki et al. (1996) | |||||
| CD68 (KP1) | Ms IgG1 | M0814 | Dako Cytomation | Pulford et al. (1989) | a,b |
| Sasaki et al. (1996) | |||||
| CD68 (PG-M1) | Ms IgG3 | M0876 | Dako Cytomation | Falini et al. (1993) | a,b |
| Rezaie et al. (2005) | |||||
| CD169 (ED3) | Ms IgG2a | MCA343R | Serotec | van den Berg et al. (1992) | |
| Ikezumi et al. (2005) | |||||
| CNPase (11-5B) | Ms IgG1 | MAB326R | Chemicon International | Kim et al. (1984) | |
| Sprinkle et al. (1987) | |||||
| CNPase (RIP) | Ms IgG | RIP | DSHB | Friedman et al. (1989) | |
| Watanabe et al. (2006) | |||||
| GFAP | Rb IgG | Z0334 | Dako Cytomation | Bignami et al. (1972) | a,b |
| Castellano et al. (1991) | |||||
| HLA-DR (LN-3) | Ms IgG2b | CM083A | Biocare Medical | Johnson et al. (1988) | |
| Swanson and Wick (1990) | |||||
| Ki67-antigen (MIB1) | Ms IgG1 | M0724 | Dako Cytomation | Gerdes et al. (1983) | a,b |
| Cattoretti et al. (1992) | |||||
| MAP-2 | Rb IgG | AB5622 | Chemicon International | Bernhardt and Matus (1984) | |
| Honig et al. (1996) | |||||
| MBP | Rb IgG | A0623 | Dako Cytomation | Johnson et al. (1988) | |
| Hardy et al. (1996) | |||||
| Nestin | Rb IgG | AB5922 | Chemicon International | Messam et al. (2000,2002) | |
| Gu et al. (2002) | |||||
| NeuN (A60) | Ms IgG1 | MAB377 | Chemicon International | Mullen et al. (1992) | |
| Lind et al. (2005) | |||||
| Neurofilament (2F11) | Ms IgG1 | M0762 | Dako Cytomation | Diepholder et al. (1991) | a,b |
| Luider et al. (1992) | |||||
| Neurofilament (DA2/FNP7/RmdO20.11) | Ms IgG1κ | 18-0171Z | Zymed | Lee et al. (1986) | a,b |
| Schmidt et al. (1990) | |||||
| NG2 | Rb IgG | AB5320 | Chemicon International | Ong and Levine (1999) | |
| Chang et al. (2000) | |||||
| Nkx-2.2 (74.6A5) | Ms IgG2b | 74.6A5 | DSHB | Qi et al. (2001) | |
| Sun et al. (2003) | |||||
| NSE (BBS / NC / VI-H14) | Ms IgG1 | M0873 | Dako Cytomation | Shimizu et al. (1983) | |
| Nogami et al. (1998) | |||||
| O4 sulfatide (81/mAB O4) | Ms IgGM | MAB345 | Chemicon International | Sommer and Schachner (1981) | |
| Bansal et al. (1989) | |||||
| PDGFα-R | Rb IgG | Sc-338 | Santa Cruz | Oumesmar et al. (1997) | |
| Zhang et al. (1999) | |||||
| p25α-antigen | Rb IgG | alfa-1 | Aarhus University | Otzen et al. (2005) | |
| Skjoerringe et al. (2006) | |||||
| S100β protein | Rb IgG | Z0311 | Dako Cytomation | Boyes et al. (1986) | a,b |
| Johnson et al. (1988) | |||||
| TOAD-64 | Rb IgG | AB5454 | Chemicon International | Minturn et al. (1995) | |
| Quinn et al. (2003) | |||||
| Vimentin (V9) | Ms IgG1 | M0725 | Dako Cytomation | Schnitzer et al. (1981) | a |
| Osborn et al. (1984) |
Antibodies are used for standardization of immunohistochemical procedures in the Nordic immunohistochemical Quality Control (NordiQC) collaboration.
Antibodies are used or standardization of immunohistochemical procedures in UK National External Quality Assessment Scheme for Immunocytochemistry (UK NEQAS).
CD45/LCA, leukocyte common antigen; CD169, sialoadhesin (CD169); CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; GFAP, glial fibrillary acidic protein; HLA-DR, HLA-DR complex; MAP-2, microtubule associated protein-2; MBP, myelin basic protein; Ms IgG, mouse immunoglobulin G; NeuN, neuronal nuclei; NG2, NG2 chondroitin sulphate proteoglycan; NSE, neuron-specific enolase; PDGFα-R, platelet-derived growth factor-α receptor; Rb IgG, rabbit immunoglobulin G; TOAD-64, turned on after division-64/Tuc-4. Suppliers: AbD Serotec, Serotec Ltd, Oxford, UK; Biocare Medical, purchased at ProHosp Denmark a/s, Værløse, Denmark; Chemicon International, purchased at AH Diagnostics, Aarhus, Denmark; Dako Cytomation, Dako Nordic a/s, Glostrup, Denmark; DSHB, Developmental Studies Hybridoma Bank, University of Iowa, Ames, IA; Novocastra Laboratories, purchased at Trichem Aps, Frederikssund, Denmark; Santa Cruz Biotechnology, Heidelberg, Germany; Stemcell Technologies SARL, Göteborg, Sweden; Zymed, San Francisco, CA. The p25α antibody was donated by Prof. P-H Jensen at The Institute of Molecular Biology, University of Aarhus, Aarhus, Denmark.