Figure 4.

aCGH mapping for MCB library 1-2 (A) shows a clear peak where the library is located on chromosome 1 starting from BAC clone RP11-106G5 to RP11-1108H17. Cross-hybridization caused by sequence homology around the centromere and 1q12 heterochromatin can also be detected at the aCGH and fluorescence in situ hybridization (FISH) level (B). Hybridization of MCB 1-2 with the array-mapped BAC clones that mark the borders of this library clearly shows the flaring effect of the FISH technique that covers a broader area than mapped by aCGH (B).