Figure 5.

Recombinant karyopherin α2-C inhibits export of in vitro imported karyopherin α2. 0.5 μM fluorescently labeled karyopherin α2, 0.3 μM karyopherin β1, 3 μM Ran, and 0.2 μM p10 were incubated in a 15 min import reaction with digitonin-permeabilized HeLa cells (8). After washing, cells were either immediatedly fixed (A) or incubated in a 30 min export reaction in the presence of 3 μM Ran (B) or 3 μM Ran and 25 μM karyopherin α2-C (C).