Table 1.
Summary statistics for anti-PSA–COIN and anti-PSA–Alexa on adjacent slides
| Detection | Analysis method | Signal/background (mean ± SD) | Spot-to-spot variation (mean ± SD) | Accuracya (mean ± SD) |
|---|---|---|---|---|
| PSA–Alexa | Image analysisb | 3 ± 1 | 43 ± 9 | 0.91 ± 0.03 |
| PSA–Alexa | Spectral analysis | 5 ± 1 | 47 ± 7 | 0.97 ± 0.01 |
| PSA–COIN | Spectral analysis | 8 ± 2 | 97 ± 19 | 0.90 ± 0.04 |
a
Fraction of correctly classified spots in the epithelium and stroma, i.e., (true positive pixels + true negative pixels)/total pixels.
b
See Materials and Methods.