Table 3.
β-galactosidase activity in yeast cells cotransformed with various combinations of fusion vectors to detect interactions between products of the het-s locus
| ORF fused the GAL4 transactivator domain | ORF fused to the
GAL4 DNA-binding domain
|
|||
|---|---|---|---|---|
| None | het-s | het-S | c-jun | |
| None | 4 (−) | 3 (−) | 3 (−) | nd |
| het-s | 4 (−) | 157 (+) | 149 (+) | nd |
| het-S | 4 (−) | 144 (+) | 155 (+) | nd |
| c-fos | nd | nd | nd | 200 (+) |
Numbers give β-galactosidase activity in units/μg of protein, (+) and (−) indicate that strains can or cannot grow on medium supplemented with 100 μg/ml of 3AT. nd, not done.