Figure 2.

Kinetics of TCR Vγ1⁺ T cells in the spleen, liver and peritoneal cavity after L. monocytogenes infection. Spleen cells, hepatic lymphocytes and plastic plate-non-adherent PEC were prepared as mentioned in the Materials and Methods from mice i.p. inoculated with 1×10³L. monocytogenes at the indicated intervals after the infection, and examined as described in Fig. 1. Percentages of Cδ⁺ cells and Vγ1⁺ cells in the CD3⁺ population are demonstrated in the upper panels (a, b and c). The cell numbers of the T-cell subsets per mouse were further calculated based on the number of lymphocytes harvested per mouse and the percentage of TCR γδ⁺ or TCR Vγ1⁺ cells, and demonstrated in the lower panels (d, e and f). The data of spleen cells (a, d), hepatic lymphocytes (b, e) and PEC (c, f) are shown. Open symbols and closed symbols represent the data of TCR γδ⁺ T cells and TCR Vγ1⁺ T cells, respectively. The data shown are the summaries of data from individual analysis of 12 mice. *P <0·05 compared to the data on day 0.