Figure 4.
Depletion of TCR Vγ1+ T cells or TCR γδ+ T cells after administration of anti-TCR Vγ1 or anti-pan TCR γδ mAb. Mice were i.p. inoculated with anti-TCR Vγ1 mAb 2·11 or anti-pan TCR γδ mAb UC-7 2 days before infection with 1×103L. monocytogenes. PEC and spleen cells were obtained on day 7 after mAb administration and stained with FITC-conjugated anti-TCR Vγ1 mAb, PE-conjugated anti-TCR γδ mAb and APC-conjugated anti-CD3 mAb as described in the Materials and Methods. All panels show the profile of TCR γδ and Vγ1 expression in CD3+ cells. The data shown are representatives of data from individual analysis of 10 mice.
