Figure 3.

CD45 antibodies inhibit the T-cell response. A panel of CD45 mAb were added to 10⁴ irradiated U937 cells, 2×10⁵ purified T cells and 0·1 μg/ml CD3 mAb (UCHT1). T-cell proliferation was assessed between 48 and 64 hr of culture by measurement of [³H]thymidine incorporation. The inhibitory response is shown as the mean percentage inhibition of proliferation in triplicate wells ±% error, when test antibody is added to the assay compared to proliferation in the absence of test antibody. Similar results have been obtained in at least two other experiments.