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. 1999 Jan;96(1):10–15. doi: 10.1046/j.1365-2567.1999.00659.x

Figure 5.

Figure 5

FACS analysis of mononuclear cells infiltrating the brain. These cells were stained with PE-conjugated anti-CD4 (GK1.5) and FITC-conjugated anti-CD8 (Lyt-2). Cells separated from the brains of five mice were analysed in each experiment. (a) Normal mice; (b) day 3 p.i.; (c) day 7 p.i.; (d) day 14 p.i.; (e) day 20 p.i.; (f) day 60 p.i.; (g) anti-CD4 mAb-treated mice on day 14 p.i.; (h) anti-CD8 mAb-treated mice on day 14 p.i.; and (i) anti-CD4 and anti-CD8 mAb-treated mice on day 14 p.i. The percentage of CD4-positive cells is shown in the upper left quadrant and at the lower right, that of CD8-positive cells. The number of T cells infiltrating the brain peaked on day 20 p.i. T cells in the brain had almost disappeared by day 60 p.i.