Figure 4.

Effect of repeated tick infestations with R. sanguineus ticks on the production of IL-4 (a) and IL-10 (b) by mice. Lymph node cells from mice infested once (1×Inf) or four times (4×Inf) with ticks were cultured (2×10⁶ cells per well) with or without Con A (2 μg/ml). After 48 hr the supernatants were collected and assayed by specific ELISA for IL-4and IL-10. *P < 0·05 and **P < 0·001 compared with cells from control mice plus Con A. The experiments were performed in duplicate and repeated three times.