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Effect of repeated tick infestations with R. sanguineus on the production of TGF-β by mice. Lymph node cells from mice infested once (1×Inf) or four times (4×Inf) with ticks were cultured (2×106 cells per well) with or without Con A (2 μg/ml). After 48 hr the supernatants were collected and assayed by specific ELISA for TGF-β. *P < 0·05 and **P < 0·001 compared with cells from control mice plus Con A. The experiments were performed in duplicate and repeated three times.
