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. 1999 Mar;96(3):473–484. doi: 10.1046/j.1365-2567.1999.00676.x

Figure 1.

Figure 1

Surface expression of major histocompatibility complex (MHC) class I molecules, Ii and human leucocyte antigen (HLA)-DR on interferon-γ (IFN-γ)-treated HT-29 cells. HT-29 cells cultured in the presence of the indicated concentrations of rIFN-γ (0–500 U/ml) for 72 hr were analysed by flow cytometry using the following mAbs: W6/32 (anti-HLA-ABC framework), BU45 (reacting with a C-terminal/extracellular determinant of all Ii isoforms) and L243 (reacting with mature HLA-DR molecules), represented by filled histograms, respectively. As a negative control, HT-29 cells were stained with the mAb VIC-Y1 (reacting with a cytoplasmic determinant of Ii, outlined histograms).