Figure 4.

Unresponsiveness is long lasting.Immobilized TCR 76 or control mAb ZP 7A were incubated overnight with H.243 T cells. Subsequently, the cells were challenged with increasing concentrations of peptide and DRB1*0401-matched APC immediately (a), or following a rest period of 2 days (b) or 7 days in the presence of IL-2 (c). Proliferation was assessed by [³H]thymidine incorporation. Each value represents the mean counts per 5 min of triplicate cultures+standard deviation.