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. 1999 Apr;96(4):517–523. doi: 10.1046/j.1365-2567.1999.00702.x

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© 1999 Blackwell Science Ltd

PMC Copyright notice

The transcription of interleukin-2 (IL-2), interleukin-4 (IL-4) and interferon-γ (IFN-γ) mRNA was measured after antigen restimulation of lymphocytes from animals vaccinated 8 weeks (a) and 14weeks (b) previously with either bacillus Calmette–Guérin (BCG) or recombinant BCG (rBCG). Units of mRNA were calculated from autoradiographs of probed blots of electrophoresed PCR product. The optical density of the product was then standardized to a control mRNA (β-actin). The data represents the mean±SE of the results from each group (n = 6).