Figure 3.

Effect of the anti-VCAM-1 mAb 5F10 on time–course of PAF-and LTB₄-induced responses. PAF or LTB₄ (both at 10⁻¹⁰ mol/site) were injected intradermally at different time-points, −3 hr, −1 hr, −0·5 hr and 0 hr, prior to the i.v. administration of the antibodies (5F10 and MOPC-21, both at 2 mg/kg), the ¹¹¹In-eosinophils and ¹²⁵I-HSA. After a 1-hr accumulation period, the animals were killed and the ¹¹¹In-eosinophil accumulation/skin site and oedema formation were quantified as described in the Materials and Methods. Panels (a) and (b) show the time–course profiles of ¹¹¹In-eosnophil accumulation induced by PAF and LTB₄, respectively, and panel (c) shows the time–course of PAF-induced oedema. The results, which have been corrected for the small level of counts detected in corresponding Tyrode-injected skin sites, are mean±SEM for (n = at least five pairs of rats). Significant differences in responses detected between MOPC-21-treated and 5F10-treated rats are shown by asterisks, *P < 0·05 and **P < 0·01.