Figure 1.

IL-2-induced human T-cell adhesion to ECM components is specifically inhibited by galectin-1. ⁵¹[Cr]-labelled human T cells were incubated for 30 min in microtitre plates pre-coated with intact ECM (a), FN (b), LN (c) and CO-IV (d) in the presence of IL-2 (10 U/ml) and the indicated concentrations of recombinant galectin-1. In some wells, T cells were also incubated in the presence of IL-2 (10 U/ml), galectin-1 (4 μg/ml) and an antibody raised against galectin-1 (diluted 1:50). Non-stimulated T cells were used as negative controls. T cell-adhesion was then analysed. Results are expressed as mean percentage±SD of bound T cells from quadruplicate wells per experimental group. One experiment representative of four is shown.