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Expression of CD40 on porcine B cells. Porcine lymphocytes from blood (PBL), spleen, mesenteric lymph nodes (MLN), tonsil, ileal Peyer’s patch (IPP) and thymus were analysed for expression of CD40 by staining with soluble CD154 (CD40 ligand)–mCD8α fusion protein, and biotin conjugate-monoclonal anti-CD8, then FITC-labelled streptavidin. The B cells were then revealed by counterstaining with PE coupled anti-porcine immunoglobulin.
