Figure 3.

Soluble CD154 rescues activated B cells from apoptosis. Positively (anti-immunoglobulin) purified MLN B cells were cultured with anti-immunoglobulin with or without soluble CD154, and [³H]TdR uptake was measured daily. (a) After 6 days the cultures were assessed for apoptosis by either SSC/FSC analysis (b,c) or DNA analysis (d.,e) in the absence (b,d) or presence (c,e) of soluble CD154. The results are the average of triplicate cultures where the standard deviation was < 10% of the mean.