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. 1999 Jun;97(2):211–218. doi: 10.1046/j.1365-2567.1999.00770.x

Figure 6.

Figure 6

Effect of cytokines on negatively purified MLN B cell cultures. The costimulatory effect of cytokines IL-2 (25–100 U/ml), IL-4, IL-13, IFN-γ (25–100 ng/ml) on CD154-MLN B cell culture was examined after 7 days treatment by [3H]TdR uptake (a). CD154 alone (without cytokine) gave an incorporation of 8170±829 c.p.m. (shown in the figure as a horizontal line). IL-4 enhanced MLN B-cell growth with CD154 (b). Figure shown as percentage cell growth (B-cell number in CD154-MLN B-cell culture with cytokine/B-cell number with CD154 alone×100). IL-4 but not IL-2 was able to induce MLN B-cell proliferation without CD154 (c). Background count (without CD154 nor cytokine) shown as a horizontal line (480 c.p.m.). The results are the average of triplicate cultures where the standard deviation was<10% of the mean.