Figure 1.
Two methods of lymphocyte gating generate similar FACS profiles.After a 10-day coculture of 106 PBMC with (or without) irradiated Daudi cells, lymphocytes were stained with FITC-conjugated anti-γ/δ-1 TCR and analysed on a flow cytometer by one of two different methods. In the top two panels, flow cytometric data were collected from 5000 unstimulated (a) or Daudi-stimulated (b) cells fitting flow cytometric characteristics of lymphocytes. In the bottom two panels, flow cytometric data were collected from 5000 unstimulated (c) or Daudi-stimulated (d) cells which excluded propidium iodine. The percentage of γδ TCR+ T cells is indicated in each panel. Similar results were obtained in two additional experiments.