The relative contribution of IgG4 to the total IgG124 antibody activity with respect to the binding of 125I-labelled Der p of plasma #178 was deduced from the comparison between Sepharose-coupled anti-IgG4 (○) and Sepharose-coupled Staphylococcal Protein A (•); the results obtained with the chimeric IgG4 antibody, 17 μg/ml, are also shown (anti-IgG4, ▵, Protein A, ▴).