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The bispecific binding is not caused by Der p I contamination of the grass pollen–Sepharose. Serial dilutions of rabbit polyclonal antiserum to Der p (○, □) and plasma #741B (•, ▪) were incubated with protein A–Sepharose (○, •) or with grass pollen–Sepharose (□, ▪). The binding was detected using radiolabelled Der p I. The results were expressed as the amount of radioactivity bound relative to the amount of radioactivity added.
