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Gel filtration of serum #741. Serum #741, 200 μl was fractionated on an ACA 34 column. The fractions were tested for total amounts of IgE (180 000 MW, broken line), Der p II-specific IgG (solid line) and for the binding in the heterologous binding assay (•). The void volume is indicated by ▾. The results are expressed as percentage of starting material.
