Figure 3.

Vaccination of B6 mice with B16/DC.GM fusion vaccine elicits a tumour-specific cytotoxic T-lymphocyte (CTL) response against B16. Groups of naive B6 mice were immunized twice, intraperitoneally (i.p.) with 5×10⁶ irradiated B16, B16.GM, DC.GM, B16/DC.GM or B16/DC cells. One week after the last vaccination, four mice from each vaccination group were killed. The splenocytes from each group of mice were harvested, pooled, restimulated with irradiated B16 tumour cells for 5 days in vitro and subsequently measured for cytotoxicity against B16 (a) or EL-4 (b) cells as targets in a standard 4-hr ⁵¹Cr-release assay.