Figure 2.

Functional tolerance of antigen (Ag)-specific T cells in Flt3-ligand (Flt3L)-treated adoptive transfer mice fed ovalbumin (OVA). Draining lymph node cells from adoptively transferred phosphate-buffered saline (PBS)- or Flt3L-treated mice were cultured with 1 mg/ml OVA and their proliferative capacity examined at 72 hr. PBS-treated mice fed 25 mg OVA had significantly reduced Ag-specific proliferative responses compared with PBS-treated mice fed PBS (*P < 0·005). Flt3L-treated mice fed OVA exhibited significantly reduced proliferative responses compared with PBS-treated mice fed either PBS (**P < 0·001) or OVA (†P < 0·05). The data are the mean±1 SEM of individual lymph node isolates from three mice per group and are representative of six experiments.