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Expression of chemokine receptor CXCR4 mRNA in epithelial cells by RT–PCR: Lane 1 Hin dIII & Eco RI λ DNA digest, lane 2 MNL, lane 3 primary type II alveolar epithelial cells, lane 4 A549, lane 5 RT4, lane 6 MCF-7, lane 7 negative control, lane 8 β-2-microglobulin fragment (A549). mRNA for CXCR4 is present in all types of epithelial cells tested. PCR data represents a typical one of four independent experiments.
