Table 1.
Bacterial strains used in this study
Strain | Relevant genotype* | Ref. or source |
---|---|---|
S. typhimurium | ||
LT2 | Wild type | J. Roth |
AK3042 | zjj-3042::Tn10dTet linked 99% to serB+ | 22 |
AK3112 | zjj-3112::Tn10dTet linked 78% to serB+ | 22 |
SA965 | leuBCD39 ara-7 HfrK17(pro-metA-ilv … purE) | 23 |
TE958 | leuA414(Am) hsdL (r−m+) serB1465::MudJ | 24 |
TT460 | pyrB692::Tn10 | 25 |
TT2661 | zcc-7::Tn10 linked 80% to put610 | 26 |
TT8371 | thr-469::MudA | 24 |
TT16813 | recD542::Tn10dCam | 27 |
MST1503 | zcc-5::Tn10 linked 50% to putP1123 | 26 |
MST1504 | zcc-4::Tn10 linked 20% to putA1146 | 28 |
MST1933 | mutS121::Tn10 | 29 |
MST3063 | leuA414 (Am) hsdL (r−m+) Fels−mutS121::Tn10 | This study |
MST3433 | ΔmutS280 recD542::Tn10dCam | This study |
MST3434 | ΔmutS281 | This study |
MST3516 | zjj-3042::Tn10dTet serB1465:MudJ | This study |
MST3517 | zjj-3112::Tn10dTet serB1465::MudJ | This study |
S. typhi | ||
Ty2 | Wild type | G. Mora |
TYT1148 | recD542::Tn10dCam | This study |
TYT1258 | ΔmutS153 | This study |
TYT1512 | ΔmutS153 recD542::Tn10dCam | This study |
TYT1513 | serB1465::MudJ | This study |
TYT1681 | serB1465::MudJ thr-469::MudA | This study |
TYT1685 | serB1465::MudJ thr-469::MudA pyrB692::Tn10 | This study |
TYT1932 | Hybrid (thr-pyrB substitution from S. typhimurium) | This study |
TYT1933 | ΔmutS284 hybrid (thr-pyrB substitution from S. typhimurium) | This study |
TYT1934 | recD542::Tn10dCam hybrid (thr-pyrB substitution from S. typhimurium) | This study |
TYT1935 | ΔmutS284 recD542::Tn10dCam hybrid (thr-pyrB substitution from S. typhimurium) | This study |
Tn10dTet refers to the transposition-defective derivative of Tn10, Tn10Δ16Δ17 (30). MudJ is an abbreviation for MudI1734, a Kanr mini-Mu derivative that forms stable lac operon fusions (31). MudA is an abbreviation for Mud1–8, an Ampr Mu derivative that forms stable lac operon fusions (32). (thr-pyrB substitution from S. typhimurium) refers to hybrid strains of S. typhi that contain a replacement of the thr-pyrB region of DNA from S. typhimurium. Genetic nomenclature is derived from the genetic map of S. typhimurium edition VIII (33).