Figure 5.
Western blot analysis of glycoproteins precipitated by CD22-Fc or Sn-Fc. Lysates prepared from 6-[3H] glucosamine-labelled MCF-7 cells were precipitated with either CD22-Fc or Sn-Fc, proteins separated by SDS-PAGE and transferred to nitrocellulose membranes. The blots were probed with either anti-MUC1 mAb SM3 or anti-CD44 mAb P2A1. Sn-Fc precipitates glycoproteins that are recognized specifically by the anti-MUC1 but not by the anti-CD44 mAb. The nature of the faint band detected with anti-MUC-1 following precipitation with CD22-Fc is unknown.