Figure 1.

Detection by RT-PCR analysis of Rac mRNA expression in guinea-pig eosinophils. (a) PCR products (406 bp) obtained using degenerate primers for Rac mRNA, and (b) Rac2-specific primers (577 bp). In both cases, lane 1, DNA ladder; lane 2, guinea-pig eosinophils; lane 3, human neutrophils; and lane 4, DNA ladder. In lanes 2 and 3, total RNA samples from 2×10⁶ cells were purified, reverse transcribed, and subjected to 30 cycles of amplification. (c) Rac2 PCR-amplified fragment sequence from guinea-pig eosinophils. Comparison of PCR product (577 bp) from guinea-pig eosinophils (GP, bold letters) corresponding to nucleotides 2–578 revealed 93% homology to human Rac2 (HU, Genbank access code no. NM_002872.1). A single underline corresponds to differences between the sequences. Sequence analysis was carried out using the BLAST sequencing facility available from the National Institutes of Health, Bethesda, MD, USA.