Figure 5.
Western blot analysis of fractions from guinea-pig eosinophils. A total of 5×107 purified eosinophils were subfractionated before stimulation (a) and after stimulation for 8 min with PMA (b). Fractions were subjected to Western blot analysis and antibodies to Rac2, RhoGDI, p47phox and p67phox were employed as described in the Materials and Methods to determine the subcellular localization of NADPH oxidase components in unstimulated and activated cells. These results are representative of three separate experiments.