Table 2.
Anti‐cytotoxic T‐lymphocyte antigen‐4 (CTLA‐4) monoclonal antibodies (mAbs) augment alloimmune response of human T cells in secondary mixed lymphocyte response (MLR)
| [3H]TdR incorporation (c.p.m. × 10–3 ± SD) | ||||||
|---|---|---|---|---|---|---|
| Day 3 | Day 4 | |||||
| Stimulator* | S : R | mAb | Isotype | Anti‐CTLA‐4 | Isotype | Anti‐CTLA‐4 |
| RPMI 8866 | 1 : 20 | IgG2a | 53·9 ± 2·4 | 17·7 ± 2·4 | ||
| 2G12 | 63·0 ± 4·4 | 21·8 ± 2·6 | ||||
| 4B10 | 64·1 ± 5·1 | 22·5 ± 2·6 | ||||
| IgG2b | 56·7 ± 1·6 | 15·9 ± 1·6 | ||||
| 1G3 | 67·3 ± 1·8 | 20·7 ± 3·2 | ||||
| 1 : 50 | IgG2a | 27·7 ± 1·0 | 7·8 ± 0·6 | |||
| 2G12 | 39·0 ± 1·9 | 9·8 ± 1·4 | ||||
| 4B10 | 34·4 ± 2·3 | 9·2 ± 2·0 | ||||
| IgG2b | 29·9 ± 2·6 | 8·7 ± 1·5 | ||||
| 1G3 | 37·1 ± 2·5 | 8·0 ± 2·5 | ||||
| KM‐H2 | 1 : 20 | IgG2a | 30·7 ± 2·4 | 26·7 ± 0·7 | ||
| 2G12 | 27·6 ± 3·7 | 29·0 ± 2·5 | ||||
| 4B10 | 31·5 ± 5·9 | 28·9 ± 4·5 | ||||
| IgG2b | 31·7 ± 2·2 | 25·5 ± 3·5 | ||||
| 1G3 | 34·8 ± 3·6 | 28·9 ± 2·1 | ||||
*
T cells were cultured for 5 days in primary mixed lymphocyte response (MLR) against irradiated RPMI 8866 cells in a stimulator–responder (S : R) ratio of 1 : 20. Subsequently they were washed and resuspended in complete medium. Forty‐eight hours later, they were restimulated with either RPMI 8866 or with a third‐party stimulator (KM‐H2) in the stimulator–responder ratio indicated, in the presence of either anti‐CTLA‐4 mAb or isotype control. [3H]‐Thymidine (TdR) incorporation on days 3 and 4 is shown as the mean± SD of quadruplicate or triplicate cultures.