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. 1999 Nov;98(3):456–463. doi: 10.1046/j.1365-2567.1999.00885.x

Figure 2.

Figure 2

Effect of HRG on the formation of IIC between human IgG and either rabbit anti‐human IgG antibodies or human RF as measured by light scattering at 350 nm. The results in (a) show the increase in absorbance as a function of time due to formation of IIC containing human IgG and rabbit anti‐human IgG (69 µg/ml) at equivalence antigen : antibody ratio for a control experiment (▪ no additions), and for experiments carried out in the presence of 22·5 (□), 45 (•), 60 (○), 75 (▴) and 150 (▵) µg/ml of human HRG. The results in (b) show the increase in absorbance as a function of time due to formation of IIC containing RF (8 IU/ml) and aggregated human IgG (120 µg/ml) for a control experiment (▪ no additions), and for experiments carried out in the presence of 37·5 (□), 75 (•), 150 (○) and 300 (▵) µg/ml of human HRG. Data are representative of three separate experiments.