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. 1999 Nov;98(3):450–455. doi: 10.1046/j.1365-2567.1999.00884.x

Figure 5.

Figure 5

Effect of extracellular pH on the binding of AIgG to monocytes, NK and K562 cells. PBMC (5 × 106/ml) were incubated for 30 min at 4° with 50 µg/ml AIgG in culture medium adjusted to pH 6·5 or 7·3. Then, cells were fixed and the binding of AIgG to monocytes and NK cells was determined by two‐colour immunofluorescence using PE‐conjugated mAb anti‐CD14 and anti‐CD56, respectively, and FITC‐conjugated F(ab′)2 rabbit IgG antihuman IgG. Binding of AIgG to K562 cells was measured after incubation of K562 cells (2 × 106/ml) and AIgG (50 µg/ml) for 30 min at 4°. After this time, cells were fixed and the binding of AIgG was determined as described above. Results are expressed as the arithmetic mean ± SEM of five to nine experiments. *Statistical significance: P < 0·05 compared to pH 7·3.