Figure 2.

Hybridization signal of an E5‐specific probe designed for its unique TCRγ gene rearrangement by Southern blot. DNA extracted from seven metastases of the patient (lanes 1–7: right axillary lymph node; left axillary lymph node; left axillary lymph node; left inguinal lymph node; right inguinal lymph node; spleen; spleen, respectively.) obtained at autopsy was used as a template for PCR amplification reactions using Primer E5‐Nspec and Primer V‐gamma‐2. The PCR products were electrophoresed for 1 hr in a 2% agarose gel and transferred on to a nylon membrane. Hybridization was performed by incubating the membrane at 40·6° overnight in 5 ml hybridization solution with 5 ng/ml of a DIG‐labelled TCR probe which was obtained from the E5 T‐cell clone by PCR amplification using primer E5‐Nspec and primer V‐gamma‐2.