Figure 3.

Numbers of vaginal lymphocytes in immunized and non‐immunized mice before and 20 hr after vaginal challenge with wild‐type herpes simplex virus type‐2 (HSV‐2). The numbers of CD4⁺, CD8⁺ and B220⁺ lymphocytes in the vaginal mucosa were relatively small in immunized and non‐immunized mice without vaginal challenge and also in non‐immunized mice after vaginal challenge. The numbers of CD4⁺ cells were not significantly different among these five groups (P = 0·23), nor were the numbers of CD8⁺ cells (P = 0·24), while there was a marginally significant difference in the numbers of B220⁺ cells (P = 0·026, five‐group analysis of variance [anova]). At 20 hr after vaginal challenge, the numbers of all three lymphocyte types in the vagina were markedly increased in the three immunized groups but not in the non‐immunized group. The numbers of CD4⁺ cells in the immune/challenged groups were not significantly different (P = 0·15), nor were the numbers of B220⁺ cells (P = 0·42), while there was a marginally significant difference in the numbers of CD8⁺ cells (P = 0·041, three‐group anova).