Table 1.

In vitro production of IL‐8 and MCP‐1 by the transformed colonic epithelial cell line Caco‐2 and freshly isolated colonic epithelial cells

	MCP‐1 production (pg/ml)		IL‐8 production (pg/ml)
Caco‐2 cells	Isolated colonic epithelial cells	Caco‐2 cells	Isolated colonic epithelial cells
No stimulus	<21	42 ± 15	314 ± 43	283 ± 34
IL‐1β (pg/ml)
1	170 ± 39	153 ± 35	1230 ± 215	1180 ± 207
10	552 ± 71	460 ± 68	4100 ± 340	4050 ± 329
50	980 ± 142	920 ± 95	4990 ± 386	4630 ± 421
100	1451 ± 168	1180 ± 109	6860 ± 634	7050 ± 711

Confluent monolayers of intestinal epithelial cells (1 × 10⁶) were exposed to increasing concentrations of IL‐1β and cultured for 24 hr. Epithelial conditioned media were then harvested, and chemokine levels were determined by ELISA. Each result is the mean ± 95% CI of triplicate cultures in one of three independent experiments that had similar results