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. 1999 Dec;98(4):630–638. doi: 10.1046/j.1365-2567.1999.00900.x

Figure 4.

Figure 4

Relative expression of gag mRNA is identical in infected mCTLA4‐Hg1 tg and non‐tg littermates. The cDNA obtained from reaction of spleen RNA samples of individual mice with RT were amplified by either defective gag‐ or HPRT‐specific primers for 30 cycles. The bands for the two products appeared on a 2% agarose gel at the expected migration points corresponding to 237 and 163 bp, respectively. Lanes 2 and 7 are, respectively, from uninfected non‐tg and tg mice. Lanes 3–6 are from infected non‐tg mice at either the 10th (lanes 3 and 4) or the 13th week (lanes 5 and 6) after viral inoculation. Lanes 8–11 are from infected tg mice at the 10th (lanes 8 and 9) or the 13th week (lanes 10 and 11) after viral inoculation. Lane 1 is a negative control for PCR (water). Lane 12 is a specificity control for PCR using J1 plasmid containing the sequence of Du5H. No amplification was obtained after PCR reaction of RNAs samples (not shown).