Table 1.

Characteristics of anticocaine monoclonal antibodies (mAb1) obtained from the spleen of BALB/c mice immunized with cocaine conjugates K1–KLH or K2–KLH

				K1–BSA		K2–BSA
Immunogen	Ab1* clones	Isotype	Affinity (Ka)§	Reactivity†	Inhibitor‡	Reactivity†	Inhibitor‡
K1–KLH	Kl–1	κγ1	5·9 × 106	+	+	–	n/a
	K1–2	κγ1	5·9 × 104	+	+	+	+
	K1–4	κγ1	3·8 × 106	+	+	–	n/a
K2–KLH	K2–1	λγ1	1·0 × 106	+	+	+	+
	K2–2	κγ1	4·0 × 107	–	n/a	+	+
	K2–3	κγ1	2·0 × 108	–	n/a	+	+
	K2–4	κγ1	6·7 × 105	+	+	+	+
	K2–5	λγ2a	2·0 × 106	+	+	+	+

^*mAb1 are identified with the designation of the cocaine hapten precursor that elicited them (K1 or K2) followed by a number (K1–1, K1–2, etc., K2–1, K2–2, etc.).

^†Reactivity was determined by enzyme-linked immunosorbent assay (ELISA) on plates coated with K1–bovine serum albumin (BSA) or K2–BSA.

^‡The inhibitor was soluble cocaine-HCl (1 mg/ml).

^§Affinity determination was performed with ELISA on plates coated with the homologous conjugate using, as inhibitor, a range of concentrations of cocaine-HCl between 500 μg/ml and 1 ng/ml.