Table 2.
Epitope specificity: reactivity and affinity of anticocaine monoclonal antibodies (mAbs), for the major cocaine metabolites
| Inhibitors* (Ka) | ||||||
|---|---|---|---|---|---|---|
| mAb1 | Ecgonine | Ecgonine methylester | Benzoyl ecgonine | Cocaethylene | Norcocaine | Cocaine |
| K1–1 | – | – | – | + (4·5 × 103)† | + (1·6 × 104) | + (5·9 × 106) |
| K1–2 | – | – | – | – | – | + (5·9 × 104) |
| K1–4 | – | – | – | + (1·2 × 104) | + (2·2 × 104) | + (3·8 × 106) |
| K2–1 | ND‡ | – | + (1·0 × 104) | + (3·0 × 104) | + (2·0 × 105) | + (1·0 × 106) |
| K2–2 | – | – | + (1·9 × 104) | + (1·1 × 106) | + (1·5 × 107) | + (4·0 × 107) |
| K2–3 | – | – | + (3·3 × 105) | + (2·0 × 107) | + (1·8 × 108) | + (2·0 × 108) |
| K2–4 | – | – | + (2·8 × 103) | + (1·4 × 105) | + (1·0 × 106) | + (6·7 × 105) |
| K2–5 | ND‡ | – | + (8·6 × 103) | + (2·4 × 104) | + (3·6 × 104) | + (2·0 × 106) |
*
The concentration range of each inhibitor was between 500 μg/ml and 1 ng/ml; the mAb1 concentrations were 100 ng/ml.
†
Affinity constant (in parenthesis) determination was performed on plates coated with the homologous conjugate.