Figure 8.

Lack of thymocyte CD1d recognition by Vα24^invt T cells. A CD1d-reactive Vα24^invt clone, DN2.B9, was cultured with the indicated stimulator cells in the presence of phorbol 12-myristate 13-acetate (PMA). Proliferation was determined by ³H-thymidine incorporation. The stimulator cells or DN2.B9 T cells were omitted from the top and bottom experiments, respectively. The response to the C1R CD1d transfectants was set at 100%. fix, light glutaraldehyde fixation; Mock, transfected with empty expression vector.