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. 2000 Jun;100(2):141–151. doi: 10.1046/j.1365-2567.2000.00035.x

Figure 4.

Figure 4

Purified B220+ bone marrow cells remain refractory to MHC-II up-regulation. Spleen cells or MACS-purified B220+ bone marrow cells were cultured (2×106/ml) with recombinant murine IL-4 (50 U/ml), goat F(ab)2 anti-µ (10 µg/ml), rat anti-mouse CD40 (10 µg/ml) or LPS (10 µg/ml) for 24 hr before being stained and analysed for B220 and class II expression by flow cytometry. Figures in the upper right quadrant indicate the MFI of class II fluorescence of the cells within that quadrant. Results shown are based on analysis of 10 000 gated events.