Figure 1.

Antigen‐stimulated expression of cytokines is down‐regulated within 24 hr. Human AC1.1 T cells obtained 1 week after antigen stimulation were stimulated with specific antigen Der p II peptide (28–40) at 5 μg/ml. Control cultures were superstimulated with PI during the last 4 hr of incubation. Secretion‐blocked, fixed and permeabilized cells were stained for TNF‐α, IL‐4 and IL‐5. Bars represent percentage of positive cells defined based on isotype‐matched controls (solid bars, antigen only; hollow bars, superstimulation with PI).