Figure 3.

Representative flow cytometric TUNEL results showing the susceptibility of lymphocytes from a MLR to apoptosis induced by treatment with anti-Fas antibodies. (a) Fluorescence histograms of lymphocytes harvested from a day 7 MLR treated with anti-Fas or control antibodies and stained by the TUNEL procedure. (b) Time course indicating the susceptibility to Fas-induced apoptosis of lymphocytes harvested at various time points during the MLR. The results are expressed as the percentage of cells induced to undergo apoptosis following ligation of the Fas antigen and represent the mean of duplicate determinations; error bars show the SEM.