Figure 4.

h2B4 modulates its own RNA expression. Purified human NK cells (1 × 10⁶) were incubated in the presence of mAb C1.7 (200 ng/ml) or hIL‐2 (20 U/ml) for various time intervals. RNA was then extracted and subjected to RT–PCR with primers amplifying sequences of the h2B4 gene and the β‐actin gene (Table 1). (a) RT–PCR analysis of h2B4. PCR products were run on a 6% polyacrylamide gel and then stained with ethidium bromide. PCR reactions were performed within the linear range of both primer sets. (b) Band intensities from (a) are normalized to β‐actin.