Figure 1.
Immune responses against OVA. All rats were immunized with OVA in Freund's complete adjuvant 1 week after serum transfer and then booster-immunized with OVA in Freund's incomplete adjuvant 5 weeks later. Solid bars, serum from rats fed OVA; hatched bars, from control-fed rats,shaded bars, rats injected i.p with a dose of native OVA corresponding to the amount in the transferred volume of serum from OVA-fed animals. (a) DTH reaction against OVA measured as increase in ear thickness 24 hr after challenge with 50 µg OVA in 20 µl PBS. The challenge was carried out 2 weeks after the primary immunization. The bars represent the mean value of eight animals in each group and the standard error is indicated. (b) In vitro proliferation of spleen cells after stimulation with OVA. Proliferative responses are expressed as the mean of the c.p.m. in separate cultures from eight animals. The cells were prepared 1 week after the booster immunization. (c) Serum IgG anti-OVA antibodies, n = 8 animals in each group. Statistical analyses were done with the Mann–Whitney U-test.