Figure 1.

Induction of human T-cell adhesion to HA by anti-CD3 mAb (a), PMA (a), various cytokines (b), and chemokines (b). Freshly purified human T cells were radiolabelled with ⁵¹Cr, seeded on HA-coated wells, and exposed to the following T-cell activators. (a) PMA (50 ng/ml) and mouse anti-human CD3 mAb (1 µg/ml). (b) the recombinant human cytokines and chemokines: TNF-α (20 ng/ml), IL-2 (50 U/ml), MIP-1β (40 ng/ml), IL-8 (40 ng/ml) and RANTES (40 ng/ml). Mean ±SD of triplicate wells is depicted for one experiment representative of five.