Figure 3.

Inhibition of IL-2-, TNF-α- (a), MIP-1β- and IL-8-induced (b) adhesion of T cells to HA by anti-CD44 mAb and cytochalasin D. The ⁵¹Cr-labelled T cells were seeded onto HA-coated wells in the presence of IL-2 (100 U/ml), TNF-α (20 ng/ml), MIP-1β (10 ng/ml), IL-8 (10 ng/ml) and the inhibitors: anti-CD44 mAb (1 µg/ml) and cytochalasin D (0·1 µm). After 3 hr at 37° in a 7·5% CO₂ humidified atmosphere, unbound cells were removed, and the adhesion of T cells to HA was determined. Mean ±SD of triplicate wells is depicted for one experiment representative of four.